Sphingobium czechense sp nov., isolated from a hexachlorocyclohexane dump site

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Publikace nespadá pod Pedagogickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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NIHARIKA Neha MOSKALÍKOVÁ Hana KAUR Jasvinder KHAN Fazlurrahman SEDLÁČKOVÁ Miroslava HAMPL Aleš DAMBORSKÝ Jiří PROKOP Zbyněk LAL Rup

Rok publikování 2013
Druh Článek v odborném periodiku
Časopis / Zdroj International Journal of Systematic and Evolutionary Microbiology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Doi http://dx.doi.org/10.1099/ijs.0.039396-0
Obor Biochemie
Klíčová slova LIN GENES; SPHINGOMONAS; IDENTIFICATION; DEGRADATION; ACID; MICROORGANISMS; PHYLOGENY; BACTERIA; STRAIN; SOILS
Popis A yellow pigmented bacterial strain, designated LL01T, was isolated from hexachlorocyclohexane (HCH)-contaminated soil in Spolana Neratovice, former Czech producer of Lindane. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL01T occupied a distinct phylogenetic position in the Sphingobium cluster, showing highest similarity with Sphingobium rhizovicinum CC-FH12-1T (98.5%). The DNA G+C content of strain LL01T was 66.1 mol%. The predominant respiratory pigment ubiquonone Q10 is present. The polar lipid profile of the strain LL01T also corresponded to those reported for other Sphingobium species (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipids), supporting its identification as member of the genus Sphingobium. Spermidine was the major polyamine observed in the cell. The results obtained after DNA-DNA hybridization, biochemical and physiological tests clearly distinguished it from closely related species of the genus Sphingobium. Therefore, strain LL01T represents a novel species of the genus Sphingobium for which the name Sphingobium czechense LL01T sp. nov. is proposed (=CCM 7979T=DSM 25410T).
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