NON-RIGID CONTOUR-BASED TEMPORAL REGISTRATION OF 2D CELL NUCLEI IMAGES USING THE NAVIER EQUATION

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SOROKIN Dmitry TEKTONIDIS Marco ROHR Karl MATULA Pavel

Rok publikování 2014
Druh Článek ve sborníku
Konference IEEE International Symposium on Biomedical Imaging: Nano to Macro
Fakulta / Pracoviště MU

Fakulta informatiky

Citace
Doi http://dx.doi.org/10.1109/ISBI.2014.6867978
Obor Využití počítačů, robotika a její aplikace
Klíčová slova Biomedical image analysis; Image sequence analysis; Contour-based registration
Popis In live cell imaging it is essential to analyze the pure motion of subnuclear proteins without influence of the cell nucleus motion and deformation which is referred to as nucleus global motion. In this work, we propose a 2D contour-based image registration approach for compensation of the global motion of the nucleus. Compared to a previous contour-based approach, our approach employs an explicit rigid registration step to compensate the nucleus translation and rotation, it uses morphological contour matching for establishing more reliable correspondences between contours in consecutive frames, and utilizes the Navier equation for more realistically modeling the nucleus deformation. Our approach was successfully applied to real live cell microscopy image sequences and an experimental comparison with an existing contour-based registration method and an intensity-based registration method has been performed.
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