The protein inhibitors of peptidases from Eudiplozoon nipponicum and their preparation in recombinant form by using the cell-free expression systems
| Autoři | |
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| Rok publikování | 2014 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | The cell-free (CF) protein expression systems are useful applications in molecular biology enabling the rapid production of lower amount of selected protein molecule. They represent the alternative to “classical” expression systems adopting the whole live cells, e.g. bacteria - Escherichia coli or yeast – Pichia pastoris. The cell-free expression methods have been used since 1960 and up to date they were significantly improved; a number of commercial CF kits is now available. The proteolytic enzymes – peptidases are essential molecules not only for helminths but also for each living organisms. They are generally participating e.g. in digestive process, in modulation of host immune reaction or in evasion of host immune response. The proteolysis is at the level of the organism well controlled process regulated by number of parasite/hosts specific molecules, including inhibitors – serpins. Number of extra- or intra-cellular serpins was identified from plants, animals, viruses, and microbes. Serpins participate in fundamental physiological processes, such as blood coagulation, fibrinolysis, inflammation, signaling, immune responses and hormone carriage. This suggested their important role in host-parasite interaction. Our work is focused on the characterization of inhibitors (serpins) of serine peptidases which were identified in Eudiplozoon nipponicum (cyprinid fish ectoparasite) transcriptome data. |
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