The Kjeldahl Method as a Primary Reference Procedure for Total Protein in Certified Reference Materials Used in Clinical Chemistry. II. Selection of Direct Kjeldahl Analysis and Its Preliminary Performance Parameters

Varování

Publikace nespadá pod Pedagogickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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VINKLÁRKOVÁ Bára CHROMÝ Vratislav ŠPRONGL Luděk BITTOVÁ Miroslava RIKANOVÁ Milena OHNÚTKOVÁ Ivana ŽALUDOVÁ Lenka

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Critical Reviews in Analytical Chemistry
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://www.tandfonline.com/doi/full/10.1080/10408347.2014.892821#abstract
Doi http://dx.doi.org/10.1080/10408347.2014.892821
Obor Analytická chemie, separace
Klíčová slova serum-based reference materials; Kjeldahl method; total protein
Popis To select a Kjeldahl procedure suitable for the determination of total protein in reference materials used in laboratory medicine, we reviewed in our previous article Kjeldahl methods adopted by clinical chemistry and found an indirect two-step analysis by total Kjeldahl nitrogen corrected for its nonprotein nitrogen and a direct analysis made on isolated protein precipitates. In this article, we compare both procedures on various reference materials. An indirect Kjeldahl method gave falsely lower results than a direct analysis. Preliminary performance parameters qualify the direct Kjeldahl analysis as a suitable primary reference procedure for the certification of total protein in reference laboratories.
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