Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment

Varování

Publikace nespadá pod Pedagogickou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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SVOBODOVÁ Markéta GUMULEC Jaromír RAUDENSKÁ Martina POLANSKÁ Hana HOLUBOVÁ Monika BALVAN Jan HUDCOVÁ Kristýna KNOPFOVÁ Lucia KIZEK René ADAM Vojtech BABULA Petr MASAŘÍK Michal

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj International Journal of Oncology
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.3892/ijo.2015.2883
Obor Onkologie a hematologie
Klíčová slova breast cancer; zinc treatment; tumour size; metallothionein; glutathione
Popis Breast cancer patients negative for the nuclear oestrogen receptor alpha have a particularly poor prognosis. Therefore, the 4T1 cell line (considered as a triple-negative model) was chosen to induce malignancy in mice. The aim of the present study was to assess if zinc ions, provided in excess, may significantly modify the process of mammary oncogenesis. Zn(II) ions were chosen because of their documented antitumour effects. Zn(II) is also known to induce the expression of metallothioneins (MT) and glutathion (GSH). A total dose of zinc sulphate per one gram of mouse weight used in the experiment was 0.15 mg. We studied the expression of MT1 MT2, TP53 and MTF-1genes and also examined the effect of the tumour on antioxidant capacity. Tumour-free mice had significantly higher expression levels of the studied genes (P<0.003). Significant differences were also revealed in the gene expression between the tissues (P<0.001). The highest expression levels were observed in the liver. As compared to brain, lung and liver, significantly lower concentrations of MT protein were found in the primary tumour; an inverse trend was observed in the concentration of Zinc(II). In non-tumour mice, the amount of hepatic hydrosulphuryl groups significantly increased by the exposure to Zn(II), but the animals with tumour induction showed no similar trend. The primary tumour size of zinc-treated animals was 20% smaller (P=0.002); however, no significant effect on metastasis progression due to the zinc treatment was discovered. In conclusion, Zn(II) itself may mute the growth of primary breast tumours especially at their early stages.
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