Classification of strain CCM 4446T as Rhodococcus degradans sp. nov.

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Publikace nespadá pod Pedagogickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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ŠVEC Pavel ČERNOHLÁVKOVÁ Jitka BUSSE Hans Jurgen VOJTKOVÁ Hana PANTŮČEK Roman CNOCKAERT Margo MAŠLAŇOVÁ Ivana KRÁLOVÁ Stanislava VANDAMME Peter SEDLÁČEK Ivo

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj International Journal of Systematic and Evolutionary Microbiology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000584
Doi http://dx.doi.org/10.1099/ijsem.0.000584
Obor Mikrobiologie, virologie
Klíčová slova Rhodococcus degradans sp. nov.; taxonomy; biodegradation
Popis Strain CCM 4446T, with notable biodegradation capabilities, was investigated in this study in order to elucidate its taxonomic position. Chemotaxonomic analyses of quinones, polar lipids, mycolic acids, polyamines and the diamino acid of the cell-wall peptidoglycan corresponded with characteristics of the genus Rhodococcus. Phylogenetic analysis, based on the 16S rRNA gene sequence, assigned strain CCM 4446T to the genus Rhodococcus and placed it in the Rhodococcus erythropolis 16S rRNA gene clade. Further analysis of catA and gyrB gene sequences, automated ribotyping with EcoRI restriction endonuclease, whole-cell protein profiling, DNA–DNA hybridization and extensive biotyping enabled differentiation of strain CCM 4446T from all phylogenetically closely related species, i.e., Rhodococcus baikonurensis, Rhodococcus qingshengii, Rhodococcus erythropolis and Rhodococcus globerulus. The results obtained show that the strain investigated represents a novel species within the genus Rhodococcus, for which the name Rhodococcus degradans sp. nov., is proposed. The type strain is CCM 4446T (LMG 28633T).
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