Allium telomeres unmasked: the unusual telomeric sequence (CTCGGTTATGGG)(n) is synthesized by telomerase

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Publikace nespadá pod Pedagogickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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FAJKUS Petr PEŠKA Vratislav ŠEDĚNKA Zdeňka FULNEČKOVÁ Jana DVOŘÁČKOVÁ Martina GOGELA Roman SÝKOROVÁ Eva HAPALA Jan FAJKUS Jiří

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj The Plant Journal
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://onlinelibrary.wiley.com/doi/10.1111/tpj.13115/abstract;jsessionid=DD9460E772BBCE39FC663F17EDC51B3F.f02t03
Doi http://dx.doi.org/10.1111/tpj.13115
Obor Genetika a molekulární biologie
Klíčová slova Allium; unusual telomere; telomerase; TERT; evolution; onion; genome sequencing
Popis Phylogenetic divergence in Asparagales plants is associated with switches in telomere sequences. The last switch occurred with divergence of the genus Allium (Amaryllidaceae) from the other Allioideae (formerly Alliaceae) genera, resulting in uncharacterized telomeres maintained by an unknown mechanism. To characterize the unknown Allium telomeres, we applied a combination of bioinformatic processing of transcriptomic and genomic data with standard approaches in telomere biology such as BAL31 sensitivity tests, terminal restriction fragment analysis, the telomere repeat amplification protocol (TRAP), and fluorescence insitu hybridization (FISH). Using these methods, we characterize the unusual telomeric sequence (CTCGGTTATGGG)(n) present in Allium species, demonstrate its synthesis by telomerase, and characterize the telomerase reverse transcriptase (TERT) subunit of Allium cepa. Our findings open up the possibility of studying the molecular details of the evolutionary genetic change in Allium telomeres and its possible role in speciation. Experimental studies addressing the implications of this change in terms of the interplay of telomere components may now be designed to shed more light on telomere functions and evolution in general.
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