STUDY OF VARIOUS PARAMETERS THAT INFLUENCE THE CONTENT OF EXHALED BREATH CONDENSATE USED IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE

Varování

Publikace nespadá pod Pedagogickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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GREGUŠ Michal ĎURČ Pavol LAČNÁ Júlia FORET František KUBÁŇ Petr

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
Doi http://dx.doi.org/10.1515/hjic-2018-0007
Klíčová slova capillary electrophoresis; exhaled breath condensate; ionic analysis; pH; gastroesophageal reflux disease
Popis In this work, various parameters that influence the ionic content and pH of exhaled breath condensate in terms of the non-invasive diagnosis of gastroesophageal reflux disease were studied. Exhaled breath condensate samples were collected using a miniature and inexpensive sampling device. Capillary electrophoresis with contactless conductometric detection was used to monitor the ionic content of exhaled breath condensate. Background electrolyte composed of 20 mM of 2-(N-Morpholino)ethanesulfonic acid, 20 mM of L-Histidine, 2 mM of 18-Crown-6 and 30 mu M of cetyltrimethylammonium bromide facilitated the rapid separation of anions and cations, both in less than 2 minutes. The possibility of contamination of the exhaled breath condensate by saliva is discussed in detail. The day-to-day repeatability (n=5) of the ionic content and pH of the exhaled breath condensate was studied and was satisfactory, reflecting mainly the physiological variability.
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