Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children

Varování

Publikace nespadá pod Pedagogickou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
Autoři

URÍK Milan HURNIK Pavel ŽIAK Dušan MACHAČ Josef ŠLAPÁK Ivo MOTYKA Oldřich JABANDŽIEV Petr

Rok publikování 2019
Druh Článek v odborném periodiku
Časopis / Zdroj International journal of pediatric otorhinolaryngology
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www http://dx.doi.org/10.1016/j.ijporl.2019.04.008
Doi http://dx.doi.org/10.1016/j.ijporl.2019.04.008
Klíčová slova Retraction pocket; Cholesteatoma; Immunohistochemistry; Chidren; Pars tensa
Popis Aims: Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. Identification of signs typical for cholesteatoma and support of retraction theory of cholesteatoma. Study design: a prospective study analysing 31 surgically removed retraction pockets. Department: University Hospital, Children's Medical Centre Methods: Retraction pockets processed by a standard process for immunohistochemical analysis. The observed findings were specified using antibodies CD45 LCA (leukocyte common antigen), CD31 (platelet endothelial cell adhesion molecule), D2-40 (marker of lymphatic endothelium), MMP9 (marker of degradation of connective tissue extracellular matrix) and Ki67 (cellular marker of proliferation). Results: All observed parameters except for MMP9 had a significantly higher incidence in retraction pocket stage III compared to stage II according to Charachon. Conclusion: We described immunohistochemical signs of retraction pocket pars tensa of tympanic membrane in children resulting in cholesteatoma. All the observed signs occur in the structure of matrix and perimatrix of cholesteatoma. A significantly higher incidence of all observed parameters except from MMP9 was proved in retraction pocket stage III, unlike in stage II. This observation proves the fact that retraction pocket is a progressive disease and is a procholesteatoma stage.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.