Structural basis for the multitasking nature of the potato virus Y coat protein

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Publikace nespadá pod Pedagogickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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KEZAR A. KAVCIC L. POLÁK Martin NOVÁČEK Jiří GUTIERREZ-AGUIRRE I. ZNIDARIC M.T. CO A. STARE K. GRUDEN K. RAVNIKAR M. PAHOVNIK D. ZAGAR E. MERZEL F. ANDERLUH G. PODOBNIK M.

Rok publikování 2019
Druh Článek v odborném periodiku
Časopis / Zdroj Science advances
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://advances.sciencemag.org/content/advances/5/7/eaaw3808.full.pdf
Doi http://dx.doi.org/10.1126/sciadv.aaw3808
Klíčová slova REAL-TIME PCR; CRYO-EM; PLANT-VIRUSES; POTYVIRUS; TERMINUS; ELECTROSTATICS; REFINEMENT; RESOLUTION; RESIDUES; PVYNTN
Popis Potato virus Y (PVY) is among the most economically important plant pathogens. Using cryoelectron microscopy, we determined the near-atomic structure of PVY's flexuous virions, revealing a previously unknown lumenal interplay between extended carboxyl-terminal regions of the coat protein units and viral RNA. RNA-coat protein interactions are crucial for the helical configuration and stability of the virion, as revealed by the unique near-atomic structure of RNA-free virus-like particles. The structures offer the first evidence for plasticity of the coat protein's amino- and carboxyl-terminal regions. Together with mutational analysis and in planta experiments, we show their crucial role in PVY infectivity and explain the ability of the coat protein to perform multiple biological tasks. Moreover, the high modularity of PVY virus-like particles suggests their potential as a new molecular scaffold for nanobiotechnological applications.
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