Metabolic activity of human embryos after thawing differs in atmosphere with different oxygen concentrations

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Publikace nespadá pod Pedagogickou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
Název česky Metabolická aktivita lidských embryí po rozmrazení se liší v atmosféře s různými koncentracemi kyslíku
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JEŠETA Michal CELÁ Andrea ŽÁKOVÁ Jana MÁDR Aleš CRHA Igor GLATZ Zdeněk KEMPISTY Bartosz VENTRUBA Pavel

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Clinical Medicine
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://www.mdpi.com/2077-0383/9/8/2609
Doi http://dx.doi.org/10.3390/jcm9082609
Klíčová slova amino acids; human embryo; in vitro cultivation; capillary electrophoresis; fluorescence detection; metabolic activity
Přiložené soubory
Popis The vitrification of human embryos is more and more frequently being utilized as a method of assisted reproduction. For this technique, gentle treatment of the embryos after thawing is crucial. In this study, the balance of amino acids released to/consumed from the cultivation media surrounding the warmed embryos was observed in the context of a cultivation environment, which was with the atmospheric oxygen concentration approximate to 20% or with a regulated oxygen level-hysiological (5%). It is the first time that total amino acid turnover in human embryos after their freezing at post compaction stages has been evaluated. During this study, progressive embryos (developed to blastocyst stage) and stagnant embryos (without developmental progression) were analyzed. It was observed that the embryos cultivated in conditions of physiological oxygen levels (5% oxygen) showed a significantly lower consumption of amino acids from the cultivation media. Progressively developing embryos also had significantly lower total amino acid turnovers (consumption and production of amino acids) when cultured in conditions with physiological oxygen levels. Based on these results it seems that a cultivation environment with a reduced oxygen concentration decreases the risk of degenerative changes in the embryos after thawing. Therefore, the cultivation of thawed embryos in an environment with physiological oxygen levels may preclude embryonal stagnation, and can support the further development of human embryos after their thawing.
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