Single-molecule visualization of human RECQ5 interactions with single-stranded DNA recombination intermediates

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Publikace nespadá pod Pedagogickou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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XUE C. Y. MOLNÁROVÁ Lucia STEINFELD J. B. ZHAO W. X. MA C. J. ŠPÍREK Mário KANIECKI K. KWON Y. BELAN Ondrej KREJČÍ Kateřina BOULTON S. J. SUNG P. GREENE E. C. KREJČÍ Lumír

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj Nucleic acids research
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://academic.oup.com/nar/article/49/1/285/6041011
Doi http://dx.doi.org/10.1093/nar/gkaa1184
Klíčová slova REPLICATION PROTEIN-A; HOMOLOGOUS RECOMBINATION; BLOOMS-SYNDROME; RAD51 FILAMENTS; ATP HYDROLYSIS; POLYMERASE-II; HRDC DOMAIN; HELICASE; SRS2; REPAIR
Popis RECQ5 is one of five RecQ helicases found in humans and is thought to participate in homologous DNA recombination by acting as a negative regulator of the recombinase protein RAD51. Here, we use kinetic and single molecule imaging methods to monitor RECQ5 behavior on various nucleoprotein complexes. Our data demonstrate that RECQ5 can act as an ATP-dependent single-stranded DNA (ssDNA) motor protein and can translocate on ssDNA that is bound by replication protein A (RPA). RECQ5 can also translocate on RAD51-coated ssDNA and readily dismantles RAD51-ssDNA filaments. RECQ5 interacts with RAD51 through protein-protein contacts, and disruption of this interface through a RECQ5-F666A mutation reduces translocation velocity by similar to 50%. However, RECQ5 readily removes the ATP hydrolysis-deficient mutant RAD51-K133R from ssDNA, suggesting that filament disruption is not coupled to the RAD51 ATP hydrolysis cycle. RECQ5 also readily removes RAD51-I287T, a RAD51 mutant with enhanced ssDNA-binding activity, from ssDNA. Surprisingly, RECQ5 can bind to double-stranded DNA (dsDNA), but it is unable to translocate. Similarly, RECQ5 cannot dismantle RAD51-bound heteroduplex joint molecules. Our results suggest that the roles of RECQ5 in genome maintenance may be regulated in part at the level of substrate specificity.
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