Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells

• Autoři: SOURALOVÁ Tereza; HOLUBCOVÁ Zuzana; KYJOVSKÁ Drahomíra; HAMPL Aleš; KOUTNÁ Irena
• Rok publikování: 2021
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Stem Cell Research
• Fakulta / Pracoviště MU: Lékařská fakulta
• www: https://www.sciencedirect.com/science/article/pii/S1873506121004219?via%3Dihub
• Doi: http://dx.doi.org/10.1016/j.scr.2021.102574
• Klíčová slova: human embryonic stem cells; Xeno- and feeder-free derivation; two sex-discordant sibling lines
• Popis: Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.

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