SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering

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Publikace nespadá pod Pedagogickou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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BENEŠOVÁ M. BERNATOVÁ S. MIKA F. POKORNÁ Z. JEŽEK J. ŠILER M. SAMEK O. RŮŽIČKA Filip REBROŠOVÁ Katarína ZEMÁNEK P. PILÁT Z.

Rok publikování 2023
Druh Článek v odborném periodiku
Časopis / Zdroj Biosensors
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://www.mdpi.com/2079-6374/13/2/182
Doi http://dx.doi.org/10.3390/bios13020182
Klíčová slova SERS-tag; Escherichia coli; sandwich immunoassay; single-cell detection
Popis Efficient separation and sensitive identification of pathogenic bacterial strains is essential for a prosperous modern society, with direct applications in medical diagnostics, drug discovery, biodefense, and food safety. We developed a fast and reliable method for antibody-based selective immobilization of bacteria from suspension onto a gold-plated glass surface, followed by detection using strain-specific antibodies linked to gold nanoparticles decorated with a reporter molecule. The reporter molecules are subsequently detected by surface-enhanced Raman spectroscopy (SERS). Such a multi-functionalized nanoparticle is called a SERS-tag. The presented procedure uses widely accessible and cheap materials for manufacturing and functionalization of the nanoparticles and the immobilization surfaces. Here, we exemplify the use of the produced SERS-tags for sensitive single-cell detection of opportunistic pathogen Escherichia coli, and we demonstrate the selectivity of our method using two other bacterial strains, Staphylococcus aureus and Serratia marcescens, as negative controls. We believe that the described approach has a potential to inspire the development of novel medical diagnostic tools for rapid identification of bacterial pathogens.
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