Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study

Varování

Publikace nespadá pod Pedagogickou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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SAMADIAN Amir KRATOCHVÍLOVÁ Monika HOKYNKOVÁ Alica ŠÍN Petr NOVÁKOVÁ Marie ŠTĚPKA Petr POKORNÁ Andrea BABULA Petr

Rok publikování 2023
Druh Článek v odborném periodiku
Časopis / Zdroj Physiological research
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://www.biomed.cas.cz/physiolres/pdf/72/72_S535.pdf
Doi http://dx.doi.org/10.33549/physiolres.935222
Klíčová slova Pressure ulcers; Debridement; Wound healing; Oxidative stress; Gene expression
Popis Pressure ulcers (PUs), also known as pressure injuries, are chronic wounds that represent potential lifelong complications. Pressure ulcers of a deep category (III and IV) are often indicated for surgical treatment - debridement and surgical reconstruction. Sharp surgical debridement is widely used in the debridement of PUs; however, the Versajet® hydrosurgery system is becoming an increasingly popular tool for tangential excision in surgery due to its numerous advantages. This work focused on the expression of selected genes, especially those associated with oxidative stress, in PUs debrided by two approaches - sharp surgical debridement and debridement using Versajet® hydrosurgery system. Expression of following genes was evaluated: NFE2L2, ACTA2, NFKB1, VEGFA, MKI67, HMOX1, HMOX2, HIF1A, and SOD2. ACTB and PSMB were used as housekeeping genes. So far, five patients have been enrolled in the study. Preliminary results suggest no significant difference in gene expression with different pressure ulcer treatment approaches except NFE2L2, despite the macroscopic differences. However, the results revealed correlations between the expression of some genes, namely HIF1A and SOD2, VEGFA and SOD2 and VEGFA and HIF1A. These results may indicate a connection between hypoxia, oxidative stress, pressure ulcer healing processes and angiogenesis.
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