Analysis of Thiocyanate in Biological Fluids by Capillary Zone Electrophoresis
| Autoři | |
|---|---|
| Rok publikování | 2001 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Journal of Chromatography A |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Biochemie |
| Klíčová slova | Capillary electrophoresis; Rhodenase; Thiocyanate; Biuological Fluids |
| Popis | A new sensitive and simple method has been developed for the determination of thiocyanate in human serum, urine and saliva. The determinations were performed in a 75 mm fused silica capillary (64.5 cm length, 56 cm effective length) using 0.1 M b-alanine - HCl (pH 3.50) as a background electrolyte, separation voltage 18 kV (negative polarity), temperature of capillary 25 oC and direct detection at 200 nm. Serum samples were 10-times diluted with deionised water and deproteinised with acetonitrile in the ratio 1 : 2. Urine and saliva samples need only 20-fold dilution with deionised water. The proposed method was successfully applied to the determination of thiocyanate in various human serum, saliva and urine samples. |
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