Elimination Voltammetry of Adenine and Cytosine Mixtures

Varování

Publikace nespadá pod Pedagogickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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TRNKOVÁ Libuše FRIML Jiří DRAČKA Oldřich

Rok publikování 2001
Druh Článek v odborném periodiku
Časopis / Zdroj Bioelectrochemistry
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Obor Elektrochemie
Klíčová slova Elimination voltammetry with linear scan; cytosine; adenine; reduction of bases; hydrogen evolution
Popis The elimination voltammetry with linear scan (EVLS) was used to study adenine and cytosine reduction signals at the mercury electrode. In comparison with the linear scan voltammetry providing only one unresolved peak, two elimination functions provide good resolution of individual peaks and significant increase of sensitivity.Two selected functions give two well resolved peaks of adenine and cytosine in a wide concentration range, while the linear sweep voltammetry gives badly resolved peaks due to the hydrogen evolution. The best resolution of peaks is observed in acetate buffer at pH 3.8 and a detection limit for both substances is 500 nM. The concentration dependence of EVLS peak heights for one substance at the constant concentration of the other substance is linear. The peak potentials differ in these elimination functions. The difference in EVLS peak potentials gives the possibility to evaluate .EVLS contributes to the resolution of cathodic signals of purine and pyrimidine bases at very negative potentials near supporting electrolyte discharge.
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