Design and Evolution of Haloalkane Dehalogenase with Enhanced Conversion of 1,2,3-Trichloropropane by Modification of Access Tunnels

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Publikace nespadá pod Pedagogickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
Název česky Design a evoluce Haloalkáne Dehalogenázy se zrychlenou přemenou 1,2,3-Trichloropropane pomocí modifikace vstupních tunelů.
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PAVLOVÁ Martina KLVAŇA Martin CHALOUPKOVÁ Radka BANÁŠ Pavel OTYEPKA Michal WADE Rebecca NAGATA Yuji DAMBORSKÝ Jiří

Rok publikování 2009
Druh Článek v odborném periodiku
Časopis / Zdroj Nature Chemical Biology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://loschmidt.chemi.muni.cz/peg/abstracts/nchemb09.html
Obor Biochemie
Klíčová slova xxx
Popis Engineering enzymes to degrade anthropogenic compounds efficiently is challenging. We obtained Rhodococcus rhodochrous haloalkane dehalogenase mutants with up to 32-fold higher activity than wild type toward the toxic, recalcitrant anthropogenic compound 1,2,3-trichloropropane (TCP) using a new strategy. We identified key residues in access tunnels connecting the buried active site with bulk solvent by rational design and randomized them by directed evolution. The most active mutant has large aromatic residues at two out of three randomized positions and two positions modified by site-directed mutagenesis. These changes apparently enhance activity with TCP by decreasing accessibility of the active site for water molecules, thereby promoting activated complex formation. Kinetic analyses confirmed that the mutations improved carbon-halogen bond cleavage and shifted the rate-limiting step to the release of products. Engineering access tunnels by combining computer-assisted protein design with directed evolution may be a valuable strategy for refining catalytic properties of enzymes with buried active sites.
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