Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons

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Publikace nespadá pod Pedagogickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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RADIMĚŘSKÝ Tomáš FROLKOVÁ Petra JANOSZOWSKÁ Dagmar DOLEJSKÁ Monika ŠVEC Pavel ROUBALOVÁ Eva ČÍKOVÁ Petra ČÍŽEK Alois LITERÁK Ivan

Rok publikování 2010
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Applied Microbiology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2010.04797.x/abstract
Obor Mikrobiologie, virologie
Klíčová slova antimicrobials; Columba livia; Czech Republic; genes; intestinal bacteria; resistance
Popis Aims: To determine the presence of antibiotic-resistant faecal Escherichia coli and Enterococcus spp. in feral pigeons (Columba livia forma domestica) in the Czech Republic. Methods and Results: Cloacal swabs of feral pigeons collected in the city of Brno in 2006 were cultivated for antibiotic-resistant E. coli. Resistance genes, class 1 and 2 integrons, and gene cassettes were detected in resistant isolates by polymerase chain reaction (PCR). The samples were also cultivated for enterococci. Species status of enterococci isolates was determined using repetitive extragenic palindromic-PCR. Resistance genes were detected in resistant enterococci by PCR. E. coli isolates were found in 203 of 247 pigeon samples. Antibiotic resistance was recorded in three (1.5%, nE. coli = 203) isolates. Using agar containing ciprofloxacin, 12 (5%, nsamples = 247) E. coli strains resistant to ciprofloxacin were isolated. No ESBL-producing E. coli isolates were detected. A total of 143 enterococci were isolated: Ent. faecalis (36 isolates), Ent. faecium (27), Ent. durans (19), Ent. hirae (17), Ent. mundtii (17), Ent. gallinarum (12), Ent. casseliflavus (12) and Ent. columbae (3). Resistance to one to four antibiotics was detected in 45 (31%) isolates. Resistances were determined by tetK, tetL, tetM, tetO, aac(6')aph(2''), ant(4')-Ia, aph(3')-IIIa, ermB, pbp5, vanA and vanC1 genes. Conclusions: Antibiotic-resistant E. coli and Enterococcus spp. occurred in feral pigeons in various prevalences. Significance and Impact of the Study: Feral pigeon should be considered a risk species for spreading in the environment antimicrobial resistant E. coli and enterococci.
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