Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen
| Název česky | Stanovení nitrátreduktasy v celých buňkách: vedlejší účinky při použití benzylviologenu |
|---|---|
| Autoři | |
| Rok publikování | 2011 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Folia Microbiologica |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://www.springerlink.com/content/u876462762641134/ |
| Doi | http://dx.doi.org/10.1007/s12223-011-0004-6 |
| Obor | Biochemie |
| Klíčová slova | nitrate reductase; artificial electron donor; nitrate transport; inhibition |
| Popis | A comparative examination of reduced methyl [MV](+) and benzyl [BV](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter. |
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