Substrate Specificity of Haloalkane Dehalogenases
| Název česky | Substrátová specifita haloalkan dehalogenáz |
|---|---|
| Autoři | |
| Rok publikování | 2011 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Biochemical Journal |
| Fakulta / Pracoviště MU | |
| Citace | |
| Doi | http://dx.doi.org/10.1042/BJ20101405 |
| Obor | Biochemie |
| Klíčová slova | substrate specificity; haloalkane dehalogenases; halogenated compounds |
| Popis | An enzyme's substrate specificity is one of its most important characteristics. The quantitative comparison of broad-specificity enzymes requires the selection of a homogenous set of substrates for experimental testing, determination of substrate specificity data, and analysis using multivariate statistics. We describe a systematic analysis of the substrate specificities of nine wild-type and four engineered haloalkane dehalogenases. The enzymes were characterized experimentally using a set of 30 substrates selected using statistical experimental design from a set of nearly 200 halogenated compounds. |
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