Analysis of tumor markers in prostate carcinoma at RNA and protein level
| Název česky | Analýza nádorových markerů karcinomu prostaty na úrovni RNA a proteinu |
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| Autoři | |
| Rok publikování | 2011 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Prostate carcinoma belongs to group of diseases with high risk of death. The incidence of this pathology is closely connected with higher age and 90% of all cases represent acinar adenocarcinoma. In this study, we focus on the differences in gene expression between tumor and non-tumor cell lines and differences in protein spectrum in serum samples of patients and control individuals. Cell lines were represented by 22Rv1 derived from androgen-dependent primary tumor from patient with bone metastases and PNT1A, immortalized line derived from normal prostatic epitheliu. We determined a level of RNA using the TaqMan gene expression assay and analysed by the comparative Ct method using b-actin. We focused on these genes: Metallothionein 1A and 2A, prostate specific antigen, alpha methylacyl-CoA racemase (AMACR) and Caveolin-1. Using electrochemical detection and ELISA technique we determined metallothionein, AMACR and caveolin-1 in cell lysates and in serum samples of patients and controls. We found significant down regulation in expression of cav-1 gene on the other hand we observed significant up regulation in expression of PSA, metallothionein 1A, 2A and AMACR in tumor cell line 22Rv1. At protein level we found significant accumulation of metallothionein in serum samples of prostate carcinoma patients. |
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