Alkaloid chelirubine and DNA: Blue and red luminescence
| Authors | |
|---|---|
| Year of publication | 2013 |
| Type | Article in Periodical |
| Magazine / Source | Talanta |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.sciencedirect.com/science/article/pii/S0039914012008764 |
| Doi | http://dx.doi.org/10.1016/j.talanta.2012.10.045 |
| Field | Analytic chemistry |
| Keywords | Alkaloid; Benzo[c]phenanthridine; Chelirubine; DNA; Intercalation; Fluorescence |
| Description | Extracts from Himalayan herb Dicranostigma lactucoides containing alkaloid chelirubine have been used for centuries in Chinese herbal medicine. We have found a new employment for the alkaloid: it can be used as a DNA fluorescent probe showing blue (free form) and red (intercalated to DNA) luminescence emission after irradiation by violet light. Besides quantification of DNA (LOD = 6 ng.ml-1) it can also be used as a supravital cell probe because chelirubine molecules can effectively enter to the living cell through the cell membrane. |
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