Alkaloid chelirubine and DNA: Blue and red luminescence

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Authors

RÁJECKÝ Michal SLANINOVÁ Iva MOKRIŠOVÁ Petra URBANOVÁ Jana PALKOVSKÝ Martin TÁBORSKÁ Eva TÁBORSKÝ Petr

Year of publication 2013
Type Article in Periodical
Magazine / Source Talanta
MU Faculty or unit

Faculty of Science

Citation
Web http://www.sciencedirect.com/science/article/pii/S0039914012008764
Doi http://dx.doi.org/10.1016/j.talanta.2012.10.045
Field Analytic chemistry
Keywords Alkaloid; Benzo[c]phenanthridine; Chelirubine; DNA; Intercalation; Fluorescence
Description Extracts from Himalayan herb Dicranostigma lactucoides containing alkaloid chelirubine have been used for centuries in Chinese herbal medicine. We have found a new employment for the alkaloid: it can be used as a DNA fluorescent probe showing blue (free form) and red (intercalated to DNA) luminescence emission after irradiation by violet light. Besides quantification of DNA (LOD = 6 ng.ml-1) it can also be used as a supravital cell probe because chelirubine molecules can effectively enter to the living cell through the cell membrane.
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