DspA from Strongylocentrotus purpuratus: the First Biochemically Characterized Haloalkane Dehalogenase of Non-Microbial Origin.

• Authors: FOŘTOVÁ Andrea; ŠEBESTOVÁ Eva; ŠTĚPÁNKOVÁ Veronika; KOUDELÁKOVÁ Táňa; PÁLKOVÁ Lenka; DAMBORSKÝ Jiří; CHALOUPKOVÁ Radka
• Year of publication: 2013
• Type: Article in Periodical
• Magazine / Source: Biochimie
• MU Faculty or unit: Faculty of Science
• Doi: http://dx.doi.org/10.1016/j.biochi.2013.07.025
• Field: Biochemistry
• Keywords: Haloalkane Dehalogenase
• Description: Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards beta-brominated alkanes. Moreover, we identified other putative dehalogenases of eukaryotic origin, representing targets for future experiments to discover dehalogenases with novel catalytic properties.

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