On Proper Simulation of Phenomena Influencing Image Formation in Fluorescence Microscopy

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Authors

SVOBODA David ULMAN Vladimír MATYSKA Luděk MAŠKA Martin BELLA Ján STEJSKAL Stanislav

Year of publication 2014
Type Article in Proceedings
Conference 2014 IEEE International Conference on Image Processing
MU Faculty or unit

Faculty of Informatics

Citation
Web http://dx.doi.org/10.1109/ICIP.2014.7025801
Doi http://dx.doi.org/10.1109/ICIP.2014.7025801
Field Use of computers, robotics and its application
Keywords Simulation; Biomedical imaging; Fluorescence microscopy; Photobleaching; Uneven illumination
Description The simulation plays an important role in biomedical image analysis as it can inherently provide large collections of image data with absolute ground truth. Compared to real image data typically annotated by some expert, the computer generated data still lacks the authenticity due to the simplifications of various natural phenomena. In this paper, we focus on simulating the photobleaching effect and the uneven illumination, being simplified or even omitted from the majority of present simulation toolkits, to considerably improve the authenticity of computer generated data.
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