PHYSICOCHEMICAL METHODS AS A SUITABLE INSTRUMENT FOR SHORT DNA FRAGMENTS STUDY
| Authors | |
|---|---|
| Year of publication | 2014 |
| Type | Article in Proceedings |
| Conference | Sborník příspěvků XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků |
| MU Faculty or unit | |
| Citation | |
| Web | Sborník příspěvků XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků |
| Field | Physical chemistry and theoretical chemistry |
| Keywords | Oligodeoxynucleotides;elimination voltammetry procedure |
| Description | Oligodeoxynucleotides (ODNs) are observed in the promoter region of the oncogens and of human telomeric DNA as a multiple repetitive C- or G- sequences. These sequences form tetraplex structures. C- rich ODNs form the so called i-motif, a hemiprotonated C-C+ base pairing structures, at slightly acidic pH. G- rich ODNs form a G-quartet, made from four guanines and stabilized by a metal kation and can be folded into a G-tetraplex. We used electrochemical methods to study the behaviour of these compounds in buffered solutions with different pH, so we can comparatively evaluate the structure and fiction of each homo-ODN for better understanding their behaviour in solutions and on the charged interface. The study was completed by circular dichroism spektra to confirm the structure. Results should provide better understanding of these compounds, so the suitable biosensor could be designed. |
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