Monitoring the influence of antibiotic exposure using Raman spectroscopy

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Authors

SAMEK Ota ZEMÁNEK Pavel BERNATOVÁ Silvie JEZEK Jan SERY Mojmir JAKL Petr ŠILER Martin RŮŽIČKA Filip

Year of publication 2014
Type Article in Proceedings
Conference Proceedings of SPIE : BIOMEDICAL VIBRATIONAL SPECTROSCOPY VI: ADVANCES IN RESEARCH AND INDUSTRY
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1117/12.2038595
Field Medical equipment
Keywords Raman spectroscopy; bacteria; antibiotics; bacteriostatic action; bactericidal action
Description Here we report on combination of the data obtained from MICs (minimum inhibitory concentrations) with information of microoragnisms fingerprint provided by Raman spectroscopy. In our feasibility study we could follow mechanisms of the bacteriostatic versus bactericidal action on biofilm-positive Staphylococcus epidermidis simply by monitoring Raman bands corresponding to DNA translating the changes introduced by selected antibiotics. The Raman spectra of Staphylococcus epidermidis treated with a bacteriostatic agent show little effect on DNA which is in contrast with the action of a bactericidal agent where decreased in dedicated Raman spectra signal strength suggests DNA fragmentation. Moreover, we demonstrate that Raman tweezers are indeed able to distinguish strains of biofilm-forming (biofilm-positive) and biofilm-negative Staphylococcus epidermidis strains using principal component analysis (PCA).
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