Raman tweezers on bacteria: Following the mechanisms of bacteriostatic versus bactericidal action

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Authors

BERNATOVÁ Silvie SAMEK Ota PILAT Zdenek SERY Mojmir JEZEK Jan JAKL Petr ŠILER Martin KRZYŽÁNEK Vladislav ZEMÁNEK Pavel HOLÁ Veronika DVOŘÁČKOVÁ Milada RŮŽIČKA Filip

Year of publication 2014
Type Article in Proceedings
Conference Proceedings of SPIE : BIOPHOTONICS: PHOTONIC SOLUTIONS FOR BETTER HEALTH CARE IV
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1117/12.2052538
Field Medical equipment
Keywords optical trapping; tweezers; Raman spectroscopy; bacteria; antibiotics action
Description Raman tweezers represents a unique method for identification of different microorganisms on the basis of Raman scattering. Raman tweezers allows us to fix and sterile manipulate with the trapped object and in the same time check the growth, viability, response to the external environment etc by Raman signal evaluating. The investigations presented here include distinction of bacteria in general(staphylococcal cells), identification of bacteria strains (biofilm-positive and biofilm-negative) by using principal component analysis (PCA) and monitoring the influence of antibiotics.
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