DETERMINATION OF TESTOSTERONE AND ITS 5 METABOLITES IN THE INCUBATION MEDIA AS A MEASURE OF CYTOCHROME P450 METABOLIC ACTIVITY

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Authors	TURJAP Miroslav DOVRTĚLOVÁ Gabriela NOSKOVÁ Kristýna ZENDULKA Ondřej JUŘICA Jan
Year of publication	2014
Type	Article in Proceedings
Conference	CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS
MU Faculty or unit	Faculty of Medicine
Citation
Field	Biochemistry
Keywords	LIQUID-CHROMATOGRAPHY
Description	The innovative HPLC method for the determination of testosterone and its five metabolites in incubation medium with rat liver microsomes is reported. The method utilizes liquid-liquid extraction procedure, with use of internal standard prednisone. Analytes are eluted within 18.7 min. The method enables reliable determination of testosterone, 7 alpha-hydroxytestosterone, 6 beta-hydroxytestosterone, 16 alpha-hydroxytestosterone, 16 beta-hydroxytestosterone, 2 beta-hydroxytestosterone with LOD in the range 1.9-3.9 nmol/L.
Related projects:	CEITEC - Central European Institute of Technology Farmakodynamické, farmakokinetické a farmakogenetické přístupy v preklinické fázi výzkumu a vývoje léčiv