LIQUID CHROMATOGRAPHIC MASS SPECTROMETRIC DETECTION OF METABOLITES OF MINOR BENZO[C]PHENANTHRIDINE ALKALOIDS
| Authors | |
|---|---|
| Year of publication | 2014 |
| Type | Article in Proceedings |
| Conference | CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | benzophenanthridine; metabolite; microsomes; mass spectrometry; liquid chromatography |
| Attached files | |
| Description | Quaternary benzo[c]phenanthridine alkaloids (QBAs) belong to a group of substances obtained from plants of families Papaveraceae, Fumariaceae and Rutaceae. While the major and commercially available sanguinarine (SA) and chelerythrine (CHE) have been involved in biological and pharmacological studies over recent years, metabolic data on minor QBAs including pentasubstituted sanguilutine (SL) and hexasubstituted macarpine (MA) are limited, due to their slow accumulation in plant material as they represent the end products in their respective biosynthetic pathways. One of the substantial parts of the pharmacokinetic and toxicity studies is determination of their metabolic fate. Enzymes of endoplasmatic reticulum, located on rat liver microsomes (RLMs), such as cytochrome P450, may serve as a simple in vitro model for metabolic studies. The metabolites can be analyzed by high performance liquid chromatography (HPLC) coupled to tandem mass spectrometry (MS). Despite the very similar structure of QBAs, metabolic pathways may differ considerably. Results published on metabolites of SA and CHE indicate demethylation, hydroxylation and ring-opening reactions. |
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