On-capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis

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Authors

GLATZ Zdeněk

Year of publication 2015
Type Article in Periodical
Magazine / Source Electrophoresis
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1002/elps.201400449
Field Biochemistry
Keywords capillary electrophoresis; derivatisation; enhancing of sensitivity; on-capillary derivatisation;
Description Separation technologies play an important role in revealing biological processes at various omic levels, in pharmacological and clinical research. In this context capillary electrophoresis (CE) is a strong candidate for analyses of samples with rapidly increasing complexity. Even though CE is well known for its many advantages in this regard, the sensitivity of CE analyses is insufficient for many applications. Accordingly, there are generally three main options for enhancing the sensitivity of CE analyses – using special detection techniques, using sample preconcentration and derivatisation. Derivatisation is often the method of choice for many laboratories, since it is simple and provides several advantages such as small sample volume demand and the possibility of automation.
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