Candida parapsilosis Biofilm Identification by Raman Spectroscopy

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Authors

SAMEK Ota MLYNÁRIKOVÁ Katarína BERNATOVÁ Silvie JEŽEK Jan KRZYŽÁNEK Vladislav ŠILER Martin ZEMÁNEK Pavel RŮŽIČKA Filip HOLÁ Veronika MAHELOVÁ Martina

Year of publication 2014
Type Article in Periodical
Magazine / Source International Journal of Molecular Sciences
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.3390/ijms151223924
Field Microbiology, virology
Keywords Raman spectroscopy; Candida parapsilosis; biofilm
Description Colonies of Candida parapsilosis on culture plates were probed directly in situ using Raman spectroscopy for rapid identification of specific strains separated by a given time intervals (up to months apart). To classify the Raman spectra, data analysis was performed using the approach of principal component analysis (PCA). The analysis of the data sets generated during the scans of individual colonies reveals that despite the inhomogeneity of the biological samples unambiguous associations to individual strains (two biofilm-positive and two biofilm-negative) could be made.
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