Characterization of the binding of antidiabetic drugs to human serum albumin by means of CE FA

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Authors	MICHALCOVÁ Lenka GLATZ Zdeněk
Year of publication	2015
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Attached files	CECE_2015_Lenka_oprava.doc
Description	The capillary electrophoresis fontal analysis (CE-FA) was used to study the affinities between selected antidiabetic drugs (tolbutamide, chlorpropamide, acetohexamide, carbutamide) and human serum albumin (HSA). The binding constant (Kb) was measured under physiological conditions. The log Kb values obtained by means of CE-FA have a good repeatability. Apparent Kb values obtained using nonlinear regression decrease in the sequence acetohexamide > tolbutamide > chlorpropamide > carbutamide. The results provide a more quantitative picture of how these drugs bind with HSA and illustrate how CE FA can be used to examine relatively complex protein drug interactions.
Related projects:	Centrum pokročilých bioanalytických technologií Inovace předmětu Metody biochemického výzkumu - laboratorní cvičení vytvořením a zavedením nové úlohy „ Stanovení vazebných konstant protein – léčivo pomocí kapilární elektroforézy“.