RATIO IMAGING OF TEMPORAL CHANGES IN LEAF APOPLASTIC PH OF VICIA FABA (L.) PLANTS UNDER DROUGHT STRESS BY FLUORESCENCE MICROSCOPY TECHNIQUE
| Authors | |
|---|---|
| Year of publication | 2015 |
| Type | Article in Proceedings |
| Conference | Influence of abiotic and biotic stresses on properties of plants 2015 |
| MU Faculty or unit | |
| Citation | |
| Field | Physiology |
| Keywords | drought stress; leaf apoplastic pH; ratio imaging; Vicia faba |
| Description | We observed temporal dynamics of changes in apoplastic pH as a general stress response signal in fully developed leaves of Vicia faba (L.) plants both in diurnal cycle and during progressive soil drying. Use of H+-sensitive fluorescence probe in combination with ratio imaging microscopy allowed in situ measurements of intact plants with fine time resolution. Gradual increase of pH in apoplast started after 2 d of drought stress simultaneously with significantly decreased soil water content whereas changes in leaf water potential occurred later – after 4d of drought stress. The maximum change of 1.32 pH units was observed in leaf apoplast after 11 d of soil drying. The changes of pH were observed both in younger (top) leaves and in older (bottom) leaves but the amplitude was bigger in older leaves. The leaf apoplastic pH proved as a useful marker of stress signaling in plants. |
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