Comparison of four methodological approaches for drug-protein binding constant determination

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Authors

NEVÍDALOVÁ Hana MICHALCOVÁ Lenka GLATZ Zdeněk

Year of publication 2016
Type Article in Proceedings
Conference Advances in chromatography and electrophoresis, Chiranal 2016
MU Faculty or unit

Faculty of Science

Citation
Web Oficiální stránky konference
Field Biochemistry
Keywords binding constant, capillary electrophoresis, isothermal titration calorimetry, circular dichroism, equilibrium dialysis
Description Drug interactions with human serum albumin (HSA) can play an important role in determining the activity, distribution, rate of excretion or metabolism of many pharmaceutical agents in the body.Within this work, four methodologies differing in their basis – equilibrium dialysis (ED), circular dichroism (CD), isothermal titration calorimetry (ITC) and capillary electrophoresis frontal analysis (CE FA) were used for the determination of diclofenac-HSA binding constant.
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