Comparison of four methodological approaches for drug-protein binding constant determination
| Authors | |
|---|---|
| Year of publication | 2016 |
| Type | Article in Proceedings |
| Conference | Advances in chromatography and electrophoresis, Chiranal 2016 |
| MU Faculty or unit | |
| Citation | |
| Web | Oficiální stránky konference |
| Field | Biochemistry |
| Keywords | binding constant, capillary electrophoresis, isothermal titration calorimetry, circular dichroism, equilibrium dialysis |
| Description | Drug interactions with human serum albumin (HSA) can play an important role in determining the activity, distribution, rate of excretion or metabolism of many pharmaceutical agents in the body.Within this work, four methodologies differing in their basis – equilibrium dialysis (ED), circular dichroism (CD), isothermal titration calorimetry (ITC) and capillary electrophoresis frontal analysis (CE FA) were used for the determination of diclofenac-HSA binding constant. |
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