Osteogenic potential of the transcription factor c-MYB

Investor logo

Warning

This publication doesn't include Faculty of Education. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

ORALOVÁ Veronika MATALOVÁ Eva KILLINGER Michael KNOPFOVÁ Lucia ŠMARDA Jan BUCHTOVÁ marcela

Year of publication 2017
Type Article in Periodical
Magazine / Source Calcified Tissue International
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1007/s00223-016-0219-2
Field Genetics and molecular biology
Keywords mineralised matrix; micromass cultures; mouse limbs; osteogenesis; PCR Array
Description Our previous findings showed the presence of c-MYB in intramembranous bones and its involvement in the chondrogenic steps of endochondral ossification, where the up-regulation of early chondrogenic markers after c-myb overexpression was observed. Since we previously detected c-MYB in osteoblasts, we aimed to analyse the localisation of c-MYB during later stages of endochondral bone formation and address its function during bone matrix production. c-MYB-positive cells were found in the chondro-osseous junction zone in osteoblasts of trabecular bone as well as deeper in the zone of ossification in cells of spongy bone. To experimentally evaluate the osteogenic potential of c-MYB during endochondral bone formation, micromasses derived from embryonic mouse limb buds were established. Nuclear c-MYB protein expression was observed in long-term micromasses, especially in the areas around nodules. c-myb overexpression induced the expression of osteogenic-related genes such as Bmp2, Comp, Csf2 and Itgb1. Moreover, alizarin red staining and osteocalcin labelling promoted mineralised matrix production in cmyb-overexpressing cultures, whereas downregulation of cmyb by siRNA reduced mineralised matrix production. In conclusion, c-Myb plays a role in the osteogenesis of long bones by inducing osteogenic genes and causing the enhancement of mineral matrix production. This action of the transcription factor c-Myb might be of interest in the future for the establishment of novel approaches to tissue regeneration.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.