Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy

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Authors

REBROŠOVÁ Katarína SILER M. SAMEK O. RŮŽIČKA Filip BERNATOVA S. JEZEK J. ZEMANEK P. HOLÁ Veronika

Year of publication 2017
Type Article in Periodical
Magazine / Source FUTURE MICROBIOLOGY
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.2217/fmb-2016-0224
Field Microbiology, virology
Keywords Raman spectroscopy; rapid diagnostics; Staphylococcus epidermidis; Staphyococcus aureus
Description Aim: Raman spectroscopy is an analytical method with a broad range of applications across multiple scientific fields. We report on a possibility to differentiate between two important Gram-positive species commonly found in clinical material - Staphylococcus aureus and Staphylococcus epidermidis - using this rapid noninvasive technique. Materials & methods: For this, we tested 87 strains, 41 of S. aureus and 46 of S. epidermidis, directly from colonies grown on a Mueller-Hinton agar plate using Raman spectroscopy. Discussion & conclusion: The method paves a way for separation of these two species even on high number of samples and therefore, it can be potentially used in clinical diagnostics.
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