COMPARISON OF FOUR METHODS USED FOR PLASMA PROTEIN-DRUG INTERACTION STUDIES
| Authors | |
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| Year of publication | 2017 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | Plasma protein-drug binding influence pharmacodynamic and pharmacokinetic properties of the drugs. It is widely known that plasma proteins transporte bound drugs in the blood but simultaneously theses interactions thus limit drug concentration available to provide pharmacological effect as well as drug disposition and efficacy. For this reasons the study of these interactions is an essential part in development of an new drugs. Reguirement to increase laboratory efficiency leads to development of an automated high-throughput assays. In this study four methods capillary electrophoresis-frontal analysis (CE-FA), isothermal titration calorimetry (ITC), circular dichroism (CD) and equilibrium dyalysis (ED) used for measuring the binding parameters of the model systems HSA-diclofenac and HSA-lidocaine were complexly compared. |
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