Výskyt protilátek proti čtyřem vybraným patogenním mikroorganizmům u volně žijících malých savců (výsledky z r. 2015)
| Title in English | The occurrence of antibodies against four selected pathogenic microorganisms in wild small mammals (results from 2015) |
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| Authors | |
| Year of publication | 2017 |
| Type | Article in Proceedings |
| Conference | VI. Kongres klinické mikrobiologie, infekčních nemocí a epidemiologie KMINE 2017 |
| MU Faculty or unit | |
| Citation | |
| Web | VI. Kongres klinické mikrobiologie, infekčních nemocí a epidemiologie KMINE 2017 |
| Field | Immunology |
| Keywords | tick-borne infections rodents and insectivores ELISA MAT |
| Description | 1955/5000 Zoonotic tick-borne infections with increasing tick activity become a more recent problem. The population of small wild mammals is an important reservoir of zoonotic pathogens. The aim of our long-term study is the monitoring (since 2010) of the occurrence of selected pathogens in wild rodents and insectivores in several localities in the Czech Republic. This paper presents the results obtained by the examination of rodents and insectivorals captured in 2015 at two locations in the Czech Republic (Moravian Karst and Poodří). From the captured individuals (n = 164), the following species were represented on both sites: Apodemus flavicollis, Apodemus sylvaticus, Clethrionomys glareolus, and Sorex araneus. In addition, Poodří was captured by the Dark-eyed Mouse (Apodemus agrarius). The obtained blood sera and heart rinses were analyzed by immunoenzyme method (ELISA) for the presence of specific antibodies against the pathogens of Coxiella burnetii and Francisella tularensis. Antibodies against leptospira interrogans s. 1 were detected in a cardiac fingerprint by the modified agglutination assay (MAT) method. Antibodies to C. burnetii were found in blood sera (11-16%) and in samples from heart rinses (3-6%) in C. glareolus (8x), A. sylvaticus ( 2x), A. flavicolis (2x) and A. agrarius (1x). Antibodies to F. tularensis were present in blood sera (5-12%) and in cardiac rinse (0 - 6%) in C. glareolus (6x), A. flavicolis (3x), A. sylvaticus (1x) A. agrarius (1x). Antibodies against L. grippotyphosa were present in 1.8% of individuals (2x A. flavicolis and 1x S. araneus) and L. pomona antibodies in 0.6% of individuals (1x A. agrarius). Compared to previous years, no significant change in prevalence occurred, there was only a slight decrease in the number of individuals infected with leptospirae and in both C. burnetii and F. tularensis there was a slight increase. |