Formation of Homogenous Interaction Partner Sample for Affinity Studies by Capillary Electrophoresis

Investor logo

Warning

This publication doesn't include Faculty of Education. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

MICHALCOVÁ Lenka NEVÍDALOVÁ Hana GLATZ Zdeněk

Year of publication 2018
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Attached files
Description The capillary electrophoresis-frontal analysis (CE-FA) is method for determination of drug-protein binding parameters in their native states without immobilization or labelling. Classical CE-FA sample preparation process uses off-line mixing reagents outside the capillary. On the other hand, CE can prepares samples directly in the capillary using by electrophoretically mediated microanalysis (EMMA) or transversal diffusion laminar flow profile (TDLFP). Both approaches lead to the other advantages such as smaller sample consumption besides full automation. The main objective of this study was to test the TDLFP methodological approach of sample preparation for CE-FA application.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.