Močové mikroRNA jako potenciální biomarkery karcinomu močového měchýře
| Title in English | Urinary microRNAs as potential biomarkers of bladder cancer |
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| Authors | |
| Year of publication | 2018 |
| Type | Article in Proceedings |
| Conference | Klinická Onkologie |
| MU Faculty or unit | |
| Citation | |
| Keywords | urinary microRNAs; biomarkers; bladder cancer |
| Description | Background: Currently, there are no urinary-based tumour markers with sufficient sensitivity and specificity to replace cystoscopy in the detection of bladder cancer (BCA). Urinary microRNAs are emerging as clinically useful class of biomarkers for early and non-invasive detection of urologic malignancies. Patients and Methods: In this study, 155 patients with BCA and 83 healthy controls were enrolled. Expression profiles of urinary miRNAs were obtained using Affymetrix miRNA microarrays and candidate miRNAs further validated in independent cohort using specific TaqMan assays and qRT-PCR method. Results: Whole-genome profiling identified miRNA signature with significantly different concentrations in urine of BCA compared to controls (P < 0.01). In the independent validation phase of the study, three miRNAs were confirmed to have significantly higher levels in urine of patients with BCA in comparison with control groups (P < 0.0001). In addition, we observed significant decrease in two miRNAs (P < 0.01) concentrations in the urinary samples collected 3 months after surgery compared to pre-operative samples. Conclusion: We identified and validated miRNAs to have significantly higher concentrations in urine of patients with BCA in comparison with controls. Our data have shown that urinary microRNAs could serve as sensitive and specific biomarkers enabling non-invasive detection of BCa. |
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