Species-specific PCR assay for the detection of Babesia odocoilei

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Authors	BURGESS Hilary J. LOCKERBIE Betty P. AYALEW Lisanework E. DIBERNARDO Antonia HRAZDILOVÁ Kristýna MODRÝ David BOLLINGER Trent K.
Year of publication	2021
Type	Article in Periodical
Magazine / Source	Journal of Veterinary Diagnostic Investigation
MU Faculty or unit	Faculty of Science
Citation
Web	https://doi.org/10.1186/s13071-017-2064-1
Doi	http://dx.doi.org/10.1177/10406387211032927
Keywords	Babesia odocoilei; cervids; PCR; specificity
Description	We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei.
Related projects:	CEITEC 2020