Synthetic long reads reconstruction using molecular barcoding

Warning

This publication doesn't include Faculty of Education. It includes Central European Institute of Technology. Official publication website can be found on muni.cz.

Authors	DAVYDOV Alexey Nikolayevich
Year of publication	2022
MU Faculty or unit	Central European Institute of Technology
Citation
Description	The project aims to implement our newly developed method for the unique molecular identifiers (UMIs) introduction into oligonucleotides. This method can be used for RNA/DNA next generation sequencing (NGS) libraries preparation. The structure of the generated sequencing libraries with the introduced UMIs allows for a downstream long-reads reconstruction and PCR error correction resulting in accurately quantified ‚phased‘ genomic sequences or full-length transcripts. The potential range of the technology applications is very broad, and includes various fields, such as medical genomics, pharmacogenomics, molecular diagnostics, cancer genomics, and others. In this project, we focused on applying the method for the analysis 16S RNA sequencing (metagenomics) and immunogenomics analysis (TCR and BCR repertoires).
Related projects:	Posílení systému komercializace výsledků VaV na Masarykově univerzitě