Detekce bakterií Leptospira interrogans s. l. u malých savců pomocí metody MAT
| Title in English | Detection of Leptospira interrogans s. l. bacteria in small mammals using the MAT method |
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| Authors | |
| Year of publication | 2024 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The presentation deals with the group of bacteria Leptospira interrogans sensu lato. This is a pathogenic group of leptospires that cause the zoonotic disease leptospirosis, which in some of its forms can be fatal to humans. In the Czech Republic, around 20 to 30 cases occur annually. The most common hosts of these bacteria are small mammals, mainly rodents. Samples obtained from small mammals were obtained from captures. In the period 2021–2023, over 200 individuals of small mammals were captured in the Moravian Karst (permitted by the Ministry of the Environment project) using the MAT method (microscopic agglutination test). This method is considered the gold standard for detecting leptospires. It is based on the reaction of all classes of antibodies from the body of the examined mammals (in this case, spleen, kidney and liver lavage from small mammals) with live leptospire strains. The following serovars occurring in the Czech Republic were used: The following serovars of Leptospira interrogans s. were used. l.: icterohaemorrhagiae; grippotyphosa; canicola; istrica; sejroe; pomona; tarassovi; bratislava; sorex jalna. So far, 75 samples have been examined. 18 of them were found to be positive at a titer of 1:100, which is 24% positivity. The most common serovars were grippotyphosa and sejroe. These serovars are described as being most frequently found in rodents. |
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