Automated Hot Solvent Extraction and HPLC Determination of Atrazine and its Degradation Products in Soil

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Authors

HRDLIČKA Aleš DOLINOVÁ Jindřiška

Year of publication 2001
Type Article in Periodical
Magazine / Source J.Liquid Chromatogr. Rel. Technol.
MU Faculty or unit

Faculty of Science

Citation
Field Analytic chemistry
Keywords Atrazine; Metabolites; Soil; Hot Solvent Extraction; HPLC
Description Automated hot solvent extraction of atrazine (ATR), desethylatrazine (DEA), and desisopropylatrazine (DIA) from soil samples has been optimized and variation of their recoveries with total organic carbon content (TOC) in samples has been studied. Four samples (20 g each) were extracted in 40 min, each one with 120 mL of dichlormethane/acetone 65:35 v/v. Extracts were cleaned-up by gel permeation chromatography and triazine fractions were analyzed by HPLC using microbore C18 column and gradient elution. Recoveries better than 98% (ATR), 93% (DEA), and 81% (DIA) were attained by extracting soils with TOC<2.5%. Limits of detection determined on dry-weight basis were 4 ng/g DEA and 2 ng/g ATR and/or DIA. RSD values were of 3.4% for ATR, 1.1% for DEA, and 3.6% for DIA. The method has been used for determination of the analytes in soil from maize fields.
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