Automated Hot Solvent Extraction and HPLC Determination of Atrazine and its Degradation Products in Soil
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Periodical |
| Magazine / Source | J.Liquid Chromatogr. Rel. Technol. |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | Atrazine; Metabolites; Soil; Hot Solvent Extraction; HPLC |
| Description | Automated hot solvent extraction of atrazine (ATR), desethylatrazine (DEA), and desisopropylatrazine (DIA) from soil samples has been optimized and variation of their recoveries with total organic carbon content (TOC) in samples has been studied. Four samples (20 g each) were extracted in 40 min, each one with 120 mL of dichlormethane/acetone 65:35 v/v. Extracts were cleaned-up by gel permeation chromatography and triazine fractions were analyzed by HPLC using microbore C18 column and gradient elution. Recoveries better than 98% (ATR), 93% (DEA), and 81% (DIA) were attained by extracting soils with TOC<2.5%. Limits of detection determined on dry-weight basis were 4 ng/g DEA and 2 ng/g ATR and/or DIA. RSD values were of 3.4% for ATR, 1.1% for DEA, and 3.6% for DIA. The method has been used for determination of the analytes in soil from maize fields. |
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