Elektrochemická detekce interakcí chinoxalinových a antracyklinových antibiotik s molekulami DNA.

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Title in English Electrochemical detection of the interactions of chinoxaline and antracykline antibiotics with DNA
Authors

HASOŇ Stanislav DVOŘÁK Jakub JELEN František VETTERL Vladimír

Year of publication 2001
Type Article in Proceedings
Conference Book of Abstracts. XXIV.dny lékařské biofyziky
MU Faculty or unit

Faculty of Science

Citation
Field Biophysics
Keywords DNA-echinomycin interactions; electrochemical impedance spectroscopy; mercury film electrodes; differential capacitance of the electrode double layer;
Description The dependence of the impedance Z and/or differential capacitance C of the electrode double layer on electrode potential E and on frequency in solutions of echinomycin with single stranded (ss) and double-helical (double stranded, ds) DNA was measured. Echinomycin gives a pseudocapacitance redox peak on the C-E curves at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. The capacitance measurements can thus distinguish with the aid of bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the electrode surface. This promise to use impedance measurements for detection of dsDNA in hybridization reactins.
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