Elicitation of tobacco cells with ergosterol activates a signal pathway including mobilisation of internal calcium

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Authors	KAŠPAROVSKÝ Tomáš MILAT ML MIKEŠ Vladimír HAVEL Ladislav
Year of publication	2003
Type	Article in Periodical
Magazine / Source	Plant Physiol Biochem
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	ergosterol; calcium channels; elicitor; Phytophthora; tobacco
Description	Ergosterol interacts with tobacco suspension (Nicotiana tabacum) cells and triggers pH changes of extracellular medium, oxidative burst and synthesis of phytoalexins. The use of several inhibitors of calcium channels (La3+, TMB-8, verapamil, ruthenium red, nifedipine) and a protein-kinase inhibitor (staurosporin) suggests that the elicitation with ergosterol includes the mobilization of internal calcium stores mediated by inositol 1,4,5-trisphosphate and serine/threonine protein kinases.
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