Molecular Mechanism of the interaction of elicitors from Phytophthora with plant cells.

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Title in English Molecular mechanism of the interaction of elicitors from Phytophthora with plant cells.
Authors

MIKEŠ Vladimír DAMBORSKY Jiří DOBEŠ Petr BLEIN Jean-Pierre

Year of publication 2002
Type Article in Proceedings
Conference Sborník XVIII Biochemického sjazdu
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords defense; Phytophthora; elicitor
Description We studied the link between elicitor and sterol carrier properties using a site directed mutagenesis and heterologous expression of cryptogein gene. Sterol binding kinetics was related to the biological effects of mutated proteins. The mutation in tyrosin-87 included in sterol binding altered specific binding to high affinity sites and biological activities. The results strongly suggest that formation of sterol elicitin complex is a requisite step before binding to specific receptor.
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