Analýza proteomu polyvalentního bakteriofága 812

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Title in English Proteome analysis of the polyvalent bacteriophage 812
Authors

EYER Luděk KONEČNÁ Hana ZDRÁHAL Zbyněk PREISLER Jan PANTŮČEK Roman DOŠKAŘ Jiří

Year of publication 2004
Type Article in Proceedings
Conference VIII. Pracovní setkání biochemiků a molekulárních biologů. Sborník abstraktů
MU Faculty or unit

Faculty of Science

Citation
Web http://orion.chemi.muni.cz/Setkani/setkani8/index.htm
Field Genetics and molecular biology
Keywords bacteriophage proteomics; MALDI-TOF; therapeutic bacteriphage; Staphylococcus bacteriophage
Description Virulent and polyvalent staphylophage 812, a member of Myoviridae family, exhibits strong lytic activity on a broad host range of Staphylococcus species, thus being a suitable candidate for treatment of staphylococcal infections. Genome of this phage is represented by linear 145 kb dsDNA. Our study was focused on genome and proteome comparison of 812 wild-type phage, its host-range mutants and related phages U16, 131 and SK311 with the aim to explain molecular basis of differences in their host-range. Comparison of the phage genomes revealed several rearrangements of DNA sequences as well as single nucleotide substitutions in different parts of the genomes. Nucleotide insertions and deletions of various size (~1 to 3 kb) were most frequently found in non-coding terminal genomic regions in phages with different host range. We tried to find possible correlations between mutational changes on DNA and proteome composition of the phages. After SDS-PAGE separation of phage virions digests 15 protein bands with the mass ranging from 15 to 80 kDa were identified. Twelve bands were excised for further analysis by in-gel digestion with consecutive MALDI-TOF MS.
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