Electrochemical determination of lead and glutathione in a plant cell culture

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Authors

VACEK Jan PETŘEK Jiří KIZEK René HAVEL Ladislav KLEJDUS Bořivoj TRNKOVÁ Libuše JELEN František

Year of publication 2004
Type Article in Periodical
Magazine / Source Bioelectrochemistry
MU Faculty or unit

Faculty of Science

Citation
Field Electrochemistry
Keywords Lead; Pb(II)-EDTA; glutathione; metallothionein; Voltammetry; Mercury electrode; Brdicka reaction
Description In this paper we established DPASV as the tool for the analysis of lead in plant cell culture. For the calibration procedure, lead in (Pb-EDTA) chelate has been used. The detection limit of lead was at 500 pM in phosphate buffer (pH 5.5), and 100 nM in prepared cells intracellular extract. For the determination of cystein-rich peptides, voltammetry in differential mode in cobalt(III)-containing ammonia buffer (Brdicka reaction) was used.
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